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rhil 11  (Qilu Pharmaceutical)

 
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    Structured Review

    Qilu Pharmaceutical rhil 11
    Median platelet counts during the treatment period. <t>rhIL-11,</t> recombinant human interleukin-11
    Rhil 11, supplied by Qilu Pharmaceutical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rhil 11/product/Qilu Pharmaceutical
    Average 86 stars, based on 1 article reviews
    rhil 11 - by Bioz Stars, 2026-06
    86/100 stars

    Images

    1) Product Images from "Hetrombopag for thrombocytopenia induced by concurrent or sequential chemoradiotherapy in patients with solid tumors: a double-cohort trial"

    Article Title: Hetrombopag for thrombocytopenia induced by concurrent or sequential chemoradiotherapy in patients with solid tumors: a double-cohort trial

    Journal: BMC Cancer

    doi: 10.1186/s12885-025-15343-x

    Median platelet counts during the treatment period. rhIL-11, recombinant human interleukin-11
    Figure Legend Snippet: Median platelet counts during the treatment period. rhIL-11, recombinant human interleukin-11

    Techniques Used: Recombinant



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    Image Search Results


    Median platelet counts during the treatment period. rhIL-11, recombinant human interleukin-11

    Journal: BMC Cancer

    Article Title: Hetrombopag for thrombocytopenia induced by concurrent or sequential chemoradiotherapy in patients with solid tumors: a double-cohort trial

    doi: 10.1186/s12885-025-15343-x

    Figure Lengend Snippet: Median platelet counts during the treatment period. rhIL-11, recombinant human interleukin-11

    Article Snippet: Cohort 1 (PLT ≥ 30 × 109/L to ≤ 50 × 109/L) received a combination of hetrombopag (Jiangsu Hengrui Pharmaceuticals Co., Ltd., China) and rhIL-11 (Shandong Qilu Pharmaceutical Co., Ltd., China), while Cohort 2 (PLT > 50 × 109/L to < 75 × 109/L) received hetrombopag monotherapy.

    Techniques: Recombinant

    ( A ) CagA induction assay. Quantitative (Q)RT-PCR analysis of IL-11 and IL-6 in WT-CagA and PR-CagA expressing MKN28 cells. Histograms show mean mRNA fold change of CagA induced cells compared to non-induced controls. Error bars show the standard error of the mean (SEM). Where present, asterisks indicate statistical significance ( P <0.05). ( B ) Immunoblot (IB) analysis of STAT3 and ERK activation levels (P-STAT3 and P-ERK) in unmodified (non-stably transfected) MKN28 cells exposed to 100 ng/mL recombinant human (rh) IL-11 or rhIL-6. Mock treated (control) cells received 0.22% saline carrier. ( C ) Quantitative immunoblot (IB) analysis of STAT3 activation in MKN28 cells exposed to 100 ng/mL IL-11 and mock-treated control cells. Histograms show mean OD values of P-STAT3 bands normalized to total STAT3 bands. Representative immunoblot images are shown (total of n = 6 replicate cultures/group). Protein molecular weights are indicated (kDa). ( D ) QRT-PCR analysis of REG3γ mRNA levels in the MKN28 cells treated with either 100 ng/mL IL-11 (same cell lysates analysed for STAT3 activation in ‘C’) or 10 ng/mL IL-6 (n = 6 replicate cultures/group). Histogram shows the mean mRNA fold-change of rhIL-11/rhIL-6 treated cells compared to mock-treated (saline carrier) controls. Error bars (±SEM). Where present, asterisks indicate statistical significance ( P <0.05).

    Journal: PLoS ONE

    Article Title: Helicobacter pylori CagA Triggers Expression of the Bactericidal Lectin REG3γ via Gastric STAT3 Activation

    doi: 10.1371/journal.pone.0030786

    Figure Lengend Snippet: ( A ) CagA induction assay. Quantitative (Q)RT-PCR analysis of IL-11 and IL-6 in WT-CagA and PR-CagA expressing MKN28 cells. Histograms show mean mRNA fold change of CagA induced cells compared to non-induced controls. Error bars show the standard error of the mean (SEM). Where present, asterisks indicate statistical significance ( P <0.05). ( B ) Immunoblot (IB) analysis of STAT3 and ERK activation levels (P-STAT3 and P-ERK) in unmodified (non-stably transfected) MKN28 cells exposed to 100 ng/mL recombinant human (rh) IL-11 or rhIL-6. Mock treated (control) cells received 0.22% saline carrier. ( C ) Quantitative immunoblot (IB) analysis of STAT3 activation in MKN28 cells exposed to 100 ng/mL IL-11 and mock-treated control cells. Histograms show mean OD values of P-STAT3 bands normalized to total STAT3 bands. Representative immunoblot images are shown (total of n = 6 replicate cultures/group). Protein molecular weights are indicated (kDa). ( D ) QRT-PCR analysis of REG3γ mRNA levels in the MKN28 cells treated with either 100 ng/mL IL-11 (same cell lysates analysed for STAT3 activation in ‘C’) or 10 ng/mL IL-6 (n = 6 replicate cultures/group). Histogram shows the mean mRNA fold-change of rhIL-11/rhIL-6 treated cells compared to mock-treated (saline carrier) controls. Error bars (±SEM). Where present, asterisks indicate statistical significance ( P <0.05).

    Article Snippet: Cell cultures were then incubated with either 100 ng/mL rhIL-11 or 10 ng/mL rhIL-6 (Sigma Aldrich) for 1 hour.

    Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Western Blot, Activation Assay, Stable Transfection, Transfection, Recombinant, Quantitative RT-PCR

    Increase in cell number after stimulation with H11. 5x10 4 Lin-CD34+ cells were cultured in X-Vivo 10 medium supplemented with rhSCF and rhIL-6 (control) and additionally in the presence of rhTPO, rhIL-11 or H11. All cytokines were used at concentration of 50 ng/ml, except of rhIL-6 which was used at 20 ng/ml. On day 7 and 14 cells were counted. Results are presented as fold increase of cell number relative to the number of cells seeded. The means and ± standard deviation of three independent experiments per cytokine combination are given.

    Journal: International Journal of Medical Sciences

    Article Title: Designer Cytokine Hyper Interleukin 11 (H11) is a Megakaryopoietic Factor

    doi: 10.7150/ijms.5638

    Figure Lengend Snippet: Increase in cell number after stimulation with H11. 5x10 4 Lin-CD34+ cells were cultured in X-Vivo 10 medium supplemented with rhSCF and rhIL-6 (control) and additionally in the presence of rhTPO, rhIL-11 or H11. All cytokines were used at concentration of 50 ng/ml, except of rhIL-6 which was used at 20 ng/ml. On day 7 and 14 cells were counted. Results are presented as fold increase of cell number relative to the number of cells seeded. The means and ± standard deviation of three independent experiments per cytokine combination are given.

    Article Snippet: The cytokines were used in the following concentrations: rhSCF 50 ng/ml (PreproTech Inc. Rocky Hill, NJ), rhIL-6 20 ng/ml (PreproTech Inc. Rocky Hill, NJ), rhTPO 50 ng/ml (PreproTech Inc. Rocky Hill, NJ), rhIL-11 50 ng/ml (Sigma, St. Louis, MO), H11 50 ng/ml.

    Techniques: Cell Culture, Concentration Assay, Standard Deviation

    Effect of H11 on the differentiation of CB Lin-CD34+ cells. Cells were seeded in X-Vivo 10 medium supplemented with rhSCF and rhIL-6 (control) plus rhTPO, rhIL-11 or H11. After 14 days of culture cells were harvested and the expression of CD41a and CD61 (A), CD34 and CD41a/61 (B), CD34 and CD235a (C), CD14 and CD15 (D) antigens was analyzed by flow cytometry. The most pronounced results of at least three times repeated experiment are shown.

    Journal: International Journal of Medical Sciences

    Article Title: Designer Cytokine Hyper Interleukin 11 (H11) is a Megakaryopoietic Factor

    doi: 10.7150/ijms.5638

    Figure Lengend Snippet: Effect of H11 on the differentiation of CB Lin-CD34+ cells. Cells were seeded in X-Vivo 10 medium supplemented with rhSCF and rhIL-6 (control) plus rhTPO, rhIL-11 or H11. After 14 days of culture cells were harvested and the expression of CD41a and CD61 (A), CD34 and CD41a/61 (B), CD34 and CD235a (C), CD14 and CD15 (D) antigens was analyzed by flow cytometry. The most pronounced results of at least three times repeated experiment are shown.

    Article Snippet: The cytokines were used in the following concentrations: rhSCF 50 ng/ml (PreproTech Inc. Rocky Hill, NJ), rhIL-6 20 ng/ml (PreproTech Inc. Rocky Hill, NJ), rhTPO 50 ng/ml (PreproTech Inc. Rocky Hill, NJ), rhIL-11 50 ng/ml (Sigma, St. Louis, MO), H11 50 ng/ml.

    Techniques: Expressing, Flow Cytometry

    H11 impact on frequency and kind of megakaryocytic colonies. Lin-CD34+ cells purified from CB were assessed for their capacity to stimulate Mk colonies formation when cultured in collagen and MegaCult-C medium containing rhIL-6, rhIL-3 (control) plus rhIL-11, H11 or rhTPO. Colonies were evaluated by immunohistochemistry (anti-CD41a/CD61) and counted under light microscopy. Results represent: A. the number of small, medium, large and platelets-like particles (PLP) rich colonies, and B. the percentage of PLP rich colonies. The means and ± standard deviations of three independent experiments per cytokine combination are shown. * p<0.05.

    Journal: International Journal of Medical Sciences

    Article Title: Designer Cytokine Hyper Interleukin 11 (H11) is a Megakaryopoietic Factor

    doi: 10.7150/ijms.5638

    Figure Lengend Snippet: H11 impact on frequency and kind of megakaryocytic colonies. Lin-CD34+ cells purified from CB were assessed for their capacity to stimulate Mk colonies formation when cultured in collagen and MegaCult-C medium containing rhIL-6, rhIL-3 (control) plus rhIL-11, H11 or rhTPO. Colonies were evaluated by immunohistochemistry (anti-CD41a/CD61) and counted under light microscopy. Results represent: A. the number of small, medium, large and platelets-like particles (PLP) rich colonies, and B. the percentage of PLP rich colonies. The means and ± standard deviations of three independent experiments per cytokine combination are shown. * p<0.05.

    Article Snippet: The cytokines were used in the following concentrations: rhSCF 50 ng/ml (PreproTech Inc. Rocky Hill, NJ), rhIL-6 20 ng/ml (PreproTech Inc. Rocky Hill, NJ), rhTPO 50 ng/ml (PreproTech Inc. Rocky Hill, NJ), rhIL-11 50 ng/ml (Sigma, St. Louis, MO), H11 50 ng/ml.

    Techniques: Purification, Cell Culture, Immunohistochemistry, Light Microscopy

    The morphology of megakaryocytic colonies. Representative pictures of small (A), medium (B) and of large (C and D) Mk-colonies are shown. Lin-CD34+ cells were cultured for 10 days in a semisolid medium supplemented with rhIL-6 and rhIL-3 (control) (A) plus rhIL-11 (B), H11 (C) or rhTPO (D). Mk cells were detected by immunohistochemistry using anti-CD41a/CD61 antibody. Pictures were taken under light microscopy (magnification 10X). Colonies cultured in the presence of H11 and TPO are examples of PLP rich colonies. Scale bar represents 100µm. The experiment was repeated three times.

    Journal: International Journal of Medical Sciences

    Article Title: Designer Cytokine Hyper Interleukin 11 (H11) is a Megakaryopoietic Factor

    doi: 10.7150/ijms.5638

    Figure Lengend Snippet: The morphology of megakaryocytic colonies. Representative pictures of small (A), medium (B) and of large (C and D) Mk-colonies are shown. Lin-CD34+ cells were cultured for 10 days in a semisolid medium supplemented with rhIL-6 and rhIL-3 (control) (A) plus rhIL-11 (B), H11 (C) or rhTPO (D). Mk cells were detected by immunohistochemistry using anti-CD41a/CD61 antibody. Pictures were taken under light microscopy (magnification 10X). Colonies cultured in the presence of H11 and TPO are examples of PLP rich colonies. Scale bar represents 100µm. The experiment was repeated three times.

    Article Snippet: The cytokines were used in the following concentrations: rhSCF 50 ng/ml (PreproTech Inc. Rocky Hill, NJ), rhIL-6 20 ng/ml (PreproTech Inc. Rocky Hill, NJ), rhTPO 50 ng/ml (PreproTech Inc. Rocky Hill, NJ), rhIL-11 50 ng/ml (Sigma, St. Louis, MO), H11 50 ng/ml.

    Techniques: Cell Culture, Immunohistochemistry, Light Microscopy